product summary
request information :
company name :
LifeSpan Biosciences
product type :
antibody
product name :
Anti-CX3CL1 / Fractalkine
catalog :
LS-C104505
quantity :
price :
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
western blot, ELISA
product information
?AntibodyID :
106355
AntibodyName :
LS-C104505
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
Anti-CX3CL1 / Fractalkine
Specificity :
Human Fractalkine
ClonalityDesc :
Polyclonal
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human Fractalkine.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in sterile water
Gene :
CX3CL1 / Fractalkine
StandardGeneSymbol :
CX3CL1
gene family :
Intercrine
Subfamily :
Intercrine delta
Reactivity :
Human
Usage :
ELISA (0.5 - 2 µg/ml), SwELISA, WB (0.1 - 0.2 µg/ml)
UsageText :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant ductal adenocarcinoma. The recommended concentration is 0.25ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: To detect hFractalkine by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Fractalkine (LS-C104345) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFractalkine. Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
CX3CL1, ABCD-3, C-X3-C motif chemokine 1, C3Xkine, Chemokine ntt, CXC3C, FKN, Fractalkine, Neurotactin, CXC3, NTN, SCYD1, Small-inducible cytokine D1
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.