This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
APOE / Apolipoprotein E Antibody (Biotin) LS-C104488
catalog :
LS-C104488
clonality :
polyclonal
host :
domestic rabbit
conjugate :
biotin
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
106338
AntibodyName :
LS-C104488
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
APOE / Apolipoprotein E Antibody (Biotin) LS-C104488
Specificity :
Human ApoE3, this antibody recognizes both ApoE2 and ApoE4 in both western blot and sandwich ELISA.
ClonalityDesc :
Polyclonal
AntibodyModification :
Biotin
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human ApoE3.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with sterile PBS and 0.1% BSA to 0.1-1.0 mg/ml
Gene :
APOE / Apolipoprotein E
StandardGeneSymbol :
APOE
Reactivity :
Human
Usage :
ELISA (0.25 - 1 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
Apolipoprotein E antibody LS-C104488 is a biotin-conjugated rabbit polyclonal antibody to human Apolipoprotein E (APOE). Validated for ELISA and WB.
UsageText :
ELISA: To detect hApoE3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hApoE3. To detect hApoE3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human ApoE3 (LS-C104471) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hApoE3. Western Blot: To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5-3 ng/lane, under either reducing or non-reducing The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms :
APOE, AD2, Apo-E, Apolipoprotein E3, Apolipoprotein E, LDLCQ5, LPG
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.