This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
FASLG / Fas Ligand Antibody LS-C104463
catalog :
LS-C104463
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
106313
AntibodyName :
LS-C104463
TargetSpecies :
Human
Host Species :
Goat
Product Name :
FASLG / Fas Ligand Antibody LS-C104463
Specificity :
Human sFas Ligand
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
CHO cell derived recombinant Human sFas Ligand.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with sterile water
Gene :
FASLG / Fas Ligand
StandardGeneSymbol :
FASLG
gene family :
TNF
Reactivity :
Human
Usage :
ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
Fas Ligand antibody LS-C104463 is an unconjugated goat polyclonal antibody to human Fas Ligand (FASLG). Validated for ELISA and WB.
UsageText :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.75 ug/mL-2.5 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a AB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of sFasL/Apo1L (10 ng/ml), a concentration of 0.08-0.1 ug/ml of this antibody is required. ELISA: To detect hsFasL/Apo1L by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sFasL/Apo1L (LS-C104462) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsFasL/Apo1L. Western Blot: To detect hsFasL/Apo1L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsFasL/Apo1L is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
FASLG, ALPS1B, Apoptosis antigen ligand, APTL, CD95L, CD95 ligand, CD178 antigen, CD95-L, Fas antigen ligand, Fas ligand, FASL, TNFSF6, APT1LG1, CD178
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.