This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CCL2 / MCP1 Antibody LS-C104459
catalog :
LS-C104459
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
106309
AntibodyName :
LS-C104459
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
CCL2 / MCP1 Antibody LS-C104459
Specificity :
Human MCP-1/MCAF
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human MCP-1/MCAF.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with sterile water
IsotypeName :
IgG1
Gene :
CCL2 / MCP1
StandardGeneSymbol :
CCL2
gene family :
Intercrine
Subfamily :
Intercrine beta
Reactivity :
Human
Usage :
ELISA (1 - 2 µg/ml), WB (0.2 - 0.4 µg/ml)
ShortWebDescription :
MCP1 antibody LS-C104459 is an unconjugated mouse monoclonal antibody to human MCP1 (CCL2). Validated for ELISA and WB.
UsageText :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 300 pg/ml of recombinant human MCP-1 when used with biotinylated antigen affinity purified anti-human MCP-1 (LS-C104657) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1-2 ng/lane, under reducing or non-reducing conditions.
Synonyms :
CCL2, C-C motif chemokine 2, Chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCP1, SCYA2, Small-inducible cytokine A2, Monocyte secretory protein JE, MCAF, MCP-1, Monocyte chemotactic protein 1, SMC-CF
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.