This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
NGF Antibody LS-C104455
catalog :
LS-C104455
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
106305
AntibodyName :
LS-C104455
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
NGF Antibody LS-C104455
Specificity :
Human beta-NGF
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from Sterile Solution PBS
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human beta-NGF.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Centrifuge vial prior to opening. Reconstitiute in sterile water to 1.0 mg/ml.
IsotypeName :
IgG2b
Gene :
NGF
StandardGeneSymbol :
NGF
gene family :
NGF Beta
Reactivity :
Human
Usage :
ELISA (2 - 4 µg/ml), WB (4 - 8 µg/ml)
ShortWebDescription :
NGF antibody LS-C104455 is an unconjugated mouse monoclonal antibody to human NGF. Validated for ELISA and WB.
UsageText :
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (10 ng/ml), a concentration of 2.7-4 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 200 pg/ml of recombinant Human beta-NGF when used with biotinylated antigen affinity purified anti-Human beta-NGF (LS-C104735) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4-8 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 4-8 ng/lane, under reducing or non-reducing conditions.
Synonyms :
NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.