This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IL6 / Interleukin 6 Antibody LS-C104444
catalog :
LS-C104444
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
106294
AntibodyName :
LS-C104444
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IL6 / Interleukin 6 Antibody LS-C104444
Specificity :
Human IL-6
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS
ImmunogenType :
Cell extract
ImmunogenDesc :
E. coli derived recombinant Human IL-6.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in sterile water to 1 mg/ml.
IsotypeName :
IgG1
Gene :
IL6 / Interleukin 6
StandardGeneSymbol :
IL6
gene family :
Hormone
Reactivity :
Human
Usage :
ELISA (1 - 2 µg/ml), WB (0.5 - 1 µg/ml)
ShortWebDescription :
Interleukin 6 antibody LS-C104444 is an unconjugated mouse monoclonal antibody to human Interleukin 6 (IL6). Validated for ELISA and WB.
UsageText :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.125 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human IL-6 when used with biotinylated antigen affinity purified anti-human IL-6 (LS-C104546) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1 ng/lane, under reducing or non-reducing conditions.
Synonyms :
IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.