product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IL17A Antibody LS-C104427
catalog :
LS-C104427
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Reference
Ma F, Plazyo O, Billi A, Tsoi L, Xing X, Wasikowski R, et al. Single cell and spatial sequencing define processes by which keratinocytes and fibroblasts amplify inflammatory responses in psoriasis. Nat Commun. 2023;14:3455 pubmed publisher
DiVito S, Aasebø A, Matos T, Hsieh P, Collin M, Elco C, et al. Peripheral host T cells survive hematopoietic stem cell transplantation and promote graft-versus-host disease. J Clin Invest. 2020;130:4624-4636 pubmed publisher
product information
AntibodyID :
106277
AntibodyName :
LS-C104427
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IL17A Antibody LS-C104427
Specificity :
Human IL-17A
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human IL-17A.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex.
Gene :
IL17A
StandardGeneSymbol :
IL17A
gene family :
Interleukin
Reactivity :
Human
Usage :
ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
IL17A antibody LS-C104427 is an unconjugated goat polyclonal antibody to human IL17A. Validated for ELISA and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml-0.25 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-17A (50.00 ng/ml), a concentration of 0.43-0.65 ug/ml of this antibody is required. ELISA: To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (LS-C104417) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
IL17A, CTLA-8, CTLA8, Interleukin-17A, Interleukin 17A, IL-17, IL-17A, IL17
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.