This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
CCL15 / MIP5 Antibody LS-C104276
catalog :
LS-C104276
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
product information
AntibodyID :
106126
AntibodyName :
LS-C104276
TargetSpecies :
Human
Host Species :
Goat
Product Name :
CCL15 / MIP5 Antibody LS-C104276
Specificity :
Human MIP-5
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human MIP-5.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with sterile water
Gene :
CCL15 / MIP5
StandardGeneSymbol :
CCL15
gene family :
Intercrine
Subfamily :
Intercrine beta
Reactivity :
Human
Usage :
ELISA (0.5 - 2 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
MIP5 antibody LS-C104276 is an unconjugated goat polyclonal antibody to human MIP5 (CCL15). Validated for ELISA and WB.
UsageText :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIP-5 (50 ng/ml), a concentration of 1.75-3 ug/ml of this antibody is required. ELISA: To detect hMIP-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-5 (LS-C104743) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-5. Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
CCL15, C-C motif chemokine 15, Chemokine hcc2, HMRP-2B, Leukotactin-1, LKN1, MIP-5, MIP5, MRP-2B, Leukotactin1, Small-inducible cytokine A15, MIP-1D, NCC3, New CC chemokine 3, SY15, Chemokine CC-2, HCC-2, Leukotactin 1, LKN-1, MIP-1 delta, NCC-3, SCYA15
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.