This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
STAT5B Antibody (aa774-787) LS-C10427
catalog :
LS-C10427
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunoprecipitation
product information
AntibodyID :
11168
AntibodyName :
LS-C10427
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
STAT5B Antibody (aa774-787) LS-C10427
Specificity :
Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa774-787
PresentationDesc :
Supplied as a liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
ImmunogenDesc :
Amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%); Panda, Bat, Chicken, Xenopus (93%); Opossum (86%).
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IsotypeName :
IgG
Gene :
STAT5B
StandardGeneSymbol :
STAT5B
Reactivity :
Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Human
Usage :
IP, WB
ShortWebDescription :
STAT5B antibody LS-C10427 is an unconjugated rabbit polyclonal antibody to STAT5B (aa774-787) from human. It is reactive with human, mouse, rat and other species. Validated for IP and WB.
UsageText :
Western Blot: 0.5-2 mg/ml detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 ug immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 ug protein) was incubated with 4 ug of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 ul of 2-mercaptoethanol. per 100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
Synonyms :
STAT5B, Transcription factor STAT5B, STAT5
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.