This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ GAD65 Antibody (aa4-22, clone N-GAD65) LS-B9984
catalog :
LS-B9984
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
N-GAD65

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, radioimmunoassay, immunohistochemistry - paraffin section
product information
AntibodyID :
238580
AntibodyName :
LS-B9984
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ GAD65 Antibody (aa4-22, clone N-GAD65) LS-B9984
Specificity :
Recognizes an epitope within the N-terminal (NT) region of Glutamate decarboxylase 2, otherwise known as GAD65/GAD2, an amphiphilic membrane-anchored protein and member of the group 2 decarboxylase family, principally expressed in the brain and also in pancreatic beta cells. GAD65 catalyzes the decarboxylation of glutamate to GABA, the major inhibitory neurotransmitter in the central nervous system. GAD65 is the 65kD isoform of GAD, encoded by the GAD2 gene, which is predominantly expressed by nerve termini, as oppose to the 67kD isoform (GAD67), which is predominantly found in the cell body, and is encoded by the GAD1 gene. Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, including Graves disease and Stiff Man Syndrome (SMS), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, and those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles. Clone N-GAD65 has been shown to be highly specific for GAD65 and does not recognize GAD67.
ClonalityDesc :
Monoclonal
CloneName :
N-GAD65
AntibodyModification :
Unconjugated
AntigenModification :
aa4-22
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Keyhole Limpet Hemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-Terminus of human GAD65. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%); Gibbon, Galago, Rat, Ferret, Panda, Bovine, Cat, Dog, Horse, Pig (95%); Monkey, Marmoset, Mouse, Rabbit, Opossum, Guinea pig (89%).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
GAD65
StandardGeneSymbol :
GAD2
Reactivity :
Human
Usage :
IHC, IHC-P (5 µg/ml), IP, RIA, WB (1:100 - 1:1000)
ShortWebDescription :
GAD65 antibody LS-B9984 is an unconjugated mouse monoclonal antibody to human GAD65 (aa4-22). Validated for IHC, IP, RIA and WB. Tested on 20 paraffin-embedded human tissues.
Synonyms :
GAD2, GAD-65, Glutamate decarboxylase 2, GAD65
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.