This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ EPCAM Antibody (clone HEA-125) LS-B9810
catalog :
LS-B9810
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HEA-125

The same clone is also sold as:
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
213161
AntibodyName :
LS-B9810
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ EPCAM Antibody (clone HEA-125) LS-B9810
Specificity :
Recognizes the 34kD cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM). This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumor specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium.
ClonalityDesc :
Monoclonal
CloneName :
HEA-125
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide, 0.5% BSA
ImmunogenType :
Cells
ImmunogenDesc :
HT29 carcinoma cell line.
PurificationDesc :
Purified IgG
RecommendedStorageDesc :
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
IsotypeName :
IgG1
Gene :
EPCAM
StandardGeneSymbol :
EPCAM
Reactivity :
Human
Usage :
Flo (1:1), IF, IHC, IHC-Fr (1:20), IHC-P (1:50)
ShortWebDescription :
EPCAM antibody LS-B9810 is an unconjugated mouse monoclonal antibody to human EPCAM. Validated for Flow, IF and IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
Flow Cytometry: Use 5 ul of the suggested working dilution to label 10^6 cells or 100 ul cell suspension. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin sections e. g. 0.1% pronase, 10 minutes. Histology Positive Control Tissue: Human colon & appendix.
Synonyms :
EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.