product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ PDIA3 / ERp57 Antibody (C-Terminus) LS-B9768
catalog :
LS-B9768
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Chamberlain N, Korwin Mihavics B, Nakada E, Bruno S, Heppner D, Chapman D, et al. Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics. Redox Biol. 2019;22:101129 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B9768 image 1
Anti-PDIA3 / ERp57 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
image 2 :
LifeSpan Biosciences LS-B9768 image 2
Western blot. Endogenous ERP57- endoplasmic reticulum lumen marker detected with PDIA3 / ERp57 antibody at 1:500 dilution. Lysates at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.
image 3 :
LifeSpan Biosciences LS-B9768 image 3
Immunofluorescence. Anti-ERP57 antibody in primary RPE cells at 1:100 dilution. Cells were fixed with 4% of PFA.
product information
AntibodyID :
212801
AntibodyName :
LS-B9768
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ PDIA3 / ERp57 Antibody (C-Terminus) LS-B9768
Specificity :
Detects a band of 60 kDa by Western blot in the following canine, human, monkey, mouse, rat whole cell lysates.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, 0.05% Sodium Azide, 20% Glycerol
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Purified recombinant peptide derived from within residues 300 aa to the C-Terminus of human ERP57 produced in E. coli.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
PDIA3 / ERp57
StandardGeneSymbol :
PDIA3
Reactivity :
Mouse, Dog, Rat, Human, Monkey
Usage :
IF (1:50 - 1:500), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000)
ShortWebDescription :
ERp57 antibody LS-B9768 is an unconjugated goat polyclonal antibody to ERp57 (PDIA3) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Synonyms :
PDIA3, 58 kDa microsomal protein, ER protein 57, ERp60, ERp61, Disulfide isomerase ER-60, Endoplasmic reticulum P58, GRP58, HsT17083, p58, Phospholipase C-alpha, PI-PLC, ER protein 60, ER60, ERp57, GRP57, Protein disulfide-isomerase A3
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.