This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MAPKAPK5 / PRAK Antibody (aa192-206) LS-B9569
catalog :
LS-B9569
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
212586
AntibodyName :
LS-B9569
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ MAPKAPK5 / PRAK Antibody (aa192-206) LS-B9569
Specificity :
Human MAPKAPK5 / PRAK
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa192-206
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-PQVLEAQRRHQKEKS, from the internal region of the protein sequence according to NP_003659.2NP_620777.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
MAPKAPK5 / PRAK
BlockingPeptide :
LS-E28034
StandardGeneSymbol :
MAPKAPK5
gene family :
Protein Kinase
Subfamily :
MAPKAPK
Reactivity :
Chicken, Zebrafish, Pig, Horse, Rat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), IP (1.5 µg/ml), Peptide-ELISA (1:64000), WB (0.5 - 1 µg/ml)
ShortWebDescription :
PRAK antibody LS-B9569 is an unconjugated goat polyclonal antibody to PRAK (MAPKAPK5) (aa192-206) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11927 or EB11928. Western blots of the IP were labeled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kD of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Synonyms :
MAPKAPK5, MAPKAP kinase 5, MAPKAP-K5, MK5, p38-regulated/activated kinase, MAPKAPK-5, PRAK, MK-5, p38-regulated protein kinase
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.