This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MAPKAPK5 / PRAK Antibody (aa306-318) LS-B9567
catalog :
LS-B9567
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
212584
AntibodyName :
LS-B9567
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ MAPKAPK5 / PRAK Antibody (aa306-318) LS-B9567
Specificity :
Human MAPKAPK5 / PRAK
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa306-318
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-STEALDNVLPSAQ, from the internal region of the protein sequence according to NP_003659.2NP_620777.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
MAPKAPK5 / PRAK
BlockingPeptide :
LS-E28033
StandardGeneSymbol :
MAPKAPK5
gene family :
Protein Kinase
Subfamily :
MAPKAPK
Reactivity :
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), IP (1.5 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 1 µg/ml)
ShortWebDescription :
PRAK antibody LS-B9567 is an unconjugated goat polyclonal antibody to PRAK (MAPKAPK5) (aa306-318) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11927 or EB11928. Western blots of the IP were labeled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kD of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Synonyms :
MAPKAPK5, MAPKAP kinase 5, MAPKAP-K5, MK5, p38-regulated/activated kinase, MAPKAPK-5, PRAK, MK-5, p38-regulated protein kinase
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.