IHC-plus™ PDCD4 Antibody (aa210-221) LS-B9458 | LifeSpan Biosciences LS-B9458 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ PDCD4 Antibody (aa210-221) LS-B9458

catalog :

LS-B9458

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human	Su Q, Li L, Zhao J, Sun Y, Yang H. Effects of Trimetazidine on PDCD4/NF-κB/TNF-α Pathway in Coronary Microembolization. Cell Physiol Biochem. 2017;42:753-760 pubmed publisher

image

image 1 :

PDCD4 antibody (0.1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

image 2 :

Anti-PDCD4 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

product information

AntibodyID :

209052

AntibodyName :

LS-B9458

TargetSpecies :

Human

Host Species :

Goat

Product Name :

IHC-plus™ PDCD4 Antibody (aa210-221) LS-B9458

Specificity :

Human PDCD4. This antibody is expected to recognize all reported isoforms (NP_055271.2; NP_663314.1; NP_001186421.1).

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

aa210-221

PresentationDesc :

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

ImmunogenDesc :

Peptide with sequence C-EGKASHREMTSK, from the internal region of the protein sequence according to NP_055271.2NP_663314.1NP_001186421.1.

PurificationDesc :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

RecommendedStorageDesc :

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Gene :

PDCD4

BlockingPeptide :

LS-E27920

StandardGeneSymbol :

PDCD4

gene family :

Apoptosis

Reactivity :

Xenopus, Chicken, Bovine, Rabbit, Mouse, Goat, Dog, Guinea pig, Bat, Rat, Hamster, Horse, Human, Monkey

Usage :

IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.1 - 0.3 µg/ml)

ShortWebDescription :

PDCD4 antibody LS-B9458 is an unconjugated goat polyclonal antibody to PDCD4 (aa210-221) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 50kD band observed in lysates of cell line HeLa (calculated MW of 50.2kD according to NP_001186421.1). Recommended concentration: 0.1-0.3 ug/ml. Tested in IHC-P only with human FFPE samples.

Synonyms :

PDCD4, H731, Nuclear antigen H731-like, Nuclear antigen H731, Protein 197/15a

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.