product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SDF1 / CXCL12 Antibody (C-Terminus) LS-B943
catalog :
LS-B943
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Reference
Martinez C, Alvarez Rodriguez M, Casado Bedmar M, Rodriguez Martinez H. In Vitro Maturation of Cumulus-Oocyte Complexes and In Vitro Sperm Capacitation Significantly Increase the Expression and Enhance the Location of the CXCL12 and CXCR4 Anchoring Attractant Complex in Pigs. Animals (Basel). 2021;11: pubmed publisher
Fricker S, Sprott K, Spyra M, Uhlig P, Lange N, David K, et al. Characterization and Validation of Antibodies for Immunohistochemical Staining of the Chemokine CXCL12. J Histochem Cytochem. 2019;67:257-266 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B943 image 1
Anti-CXCL12 antibody IHC of human breast, fibroblasts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
33933
AntibodyName :
LS-B943
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SDF1 / CXCL12 Antibody (C-Terminus) LS-B943
Specificity :
Human CXCL12 beta isoform
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from C-Terminus of human CXCL12 (P48061, NP_000600). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Dog, Cat, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%); Rat, Bovine (90%); Elephant (81%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Gene :
SDF1 / CXCL12
BlockingPeptide :
LS-E10739
StandardGeneSymbol :
CXCL12
gene family :
Intercrine
Subfamily :
Intercrine alpha
Reactivity :
Xenopus, Chicken, Mouse, Dog, Guinea pig, Bat, Horse, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
ShortWebDescription :
CXCL12 antibody LS-B943 is an unconjugated rabbit polyclonal antibody to CXCL12 (SDF1) (C-Terminus) from human. It is reactive with human, mouse, bat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B943 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B943 was determined to be 5 ug/ml.
Synonyms :
CXCL12, C-X-C motif chemokine 12, HIRH, IRH, SDF-1, SDF-1b, SDF1A, SDF1B, TPAR1, TLSF-a, TLSF-b, SDF-1a, SDF1, Sdf1alpha, Stromal cell-derived factor 1, TLSF, HSDF-1, PBSF, SCYB12
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.