product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ RPE65 Antibody (clone 401.8B11.3D9) LS-B90
catalog :
LS-B90
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
401.8B11.3D9
reactivity :
human, bovine
application :
western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Machalinska A, Kawa M, Pius Sadowska E, Rogińska D, Kłos P, Baumert B, et al. Endogenous regeneration of damaged retinal pigment epithelium following low dose sodium iodate administration: an insight into the role of glial cells in retinal repair. Exp Eye Res. 2013;112:68-78 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B90 image 1
Anti-RPE65 antibody IHC of human brain cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
27130
AntibodyName :
LS-B90
TargetSpecies :
Bovine
Host Species :
Mouse
Product Name :
PathPlus™ RPE65 Antibody (clone 401.8B11.3D9) LS-B90
Specificity :
RPE65.
ClonalityDesc :
Monoclonal
CloneName :
401.8B11.3D9
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, 0.02% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Bovine RPE microsomal membrane proteins.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
RPE65
StandardGeneSymbol :
RPE65
Reactivity :
Chicken, Bovine, Mouse, Dog, Rat, Pig, Human
Usage :
Flo, ICC, IHC-P (2.5 - 5 µg/ml), WB (1:5000 - 1:10000)
ShortWebDescription :
RPE65 antibody LS-B90 is an unconjugated mouse monoclonal antibody to RPE65 from bovine. It is reactive with human, mouse, rat and other species. Validated for Flow, ICC, IHC and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B90 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B90 was determined to be 2.5 ug/ml.
Synonyms :
RPE65, LCA2, RBP-binding membrane protein, Retinol isomerase, SRPE65, Rd12, MRPE65, Retinoid isomerohydrolase, RP20
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.