product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ POU5F1 / OCT4 Antibody (aa100-200) LS-B85
catalog :
LS-B85
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
|
image
image 1 :
Anti-POU5F1 / OCT4 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
27125
AntibodyName :
LS-B85
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ POU5F1 / OCT4 Antibody (aa100-200) LS-B85
Specificity :
OCT4 - Embryonic Stem Cell Marker.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa100-200
PresentationDesc :
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A synthetic peptide made to an internal portion of the human OCT4 protein sequence (between residues 100-200). Percent identity by BLAST analysis: Bovine, Cat, Porcine (93%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Gene :
POU5F1 / OCT4
StandardGeneSymbol :
POU5F1
Reactivity :
Mouse, Porcine, Human
Usage :
Flo (1:50 - 1:200), ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC, IHC-Fr, IHC-P (2.5 µg/ml), WB (2 µg/ml)
ShortWebDescription :
OCT4 antibody LS-B85 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) (aa100-200) from human. It is reactive with human, mouse and porcine. Validated for Flow, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B85 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B85 was determined to be 2.5 ug/ml.
Synonyms :
POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3, OCT3/4
SalesRegion :
Worldwide
more info or order :
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
related products
browse more products
questions and comments