This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ GDF15 Antibody (C-Terminus, clone 6D12.H10.E4) LS-B8327
catalog :
LS-B8327
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
6D12.H10.E4

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
162805
AntibodyName :
LS-B8327
TargetSpecies :
Human
Host Species :
Rat
Product Name :
IHC-plus™ GDF15 Antibody (C-Terminus, clone 6D12.H10.E4) LS-B8327
Specificity :
This antibody reacts with the C-terminus of endogenous NAG-1 protein from mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
ClonalityDesc :
Monoclonal
CloneName :
6D12.H10.E4
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein.  Cross reactivity to MBP is negative.
PurificationDesc :
Protein G affinity chromatography
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
IsotypeName :
IgG2a,l
Gene :
GDF15
StandardGeneSymbol :
GDF15
gene family :
TGF beta
Reactivity :
Mouse, Human
Usage :
ELISA (1:2000), IHC, IHC-P (10 µg/ml), WB (1:1000)
ShortWebDescription :
GDF15 antibody LS-B8327 is an unconjugated rat monoclonal antibody to GDF15 (C-Terminus) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
This Protein G purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately 13 and 26 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.
Synonyms :
GDF15, MIC1, MIC-1, NAG-1, NRG-1, NSAID-regulated gene 1 protein, PLAB, NSAID-activated gene 1 protein, Placental TGF-beta, PTGFB, GDF-15, PTGF-beta
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.