This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CRISP2 / TSP1 Antibody (aa81-93) LS-B8317
catalog :
LS-B8317
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
162795
AntibodyName :
LS-B8317
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ CRISP2 / TSP1 Antibody (aa81-93) LS-B8317
Specificity :
Human CRISP2. Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa81-93
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-QHSDPEDRKTSTR, from the internal region of the protein sequence according to NP_003287.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
CRISP2 / TSP1
BlockingPeptide :
LS-E27290
StandardGeneSymbol :
CRISP2
Reactivity :
Human
Usage :
IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:8000), WB (0.5 - 1 µg/ml)
ShortWebDescription :
TSP1 antibody LS-B8317 is an unconjugated goat polyclonal antibody to human TSP1 (CRISP2) (aa81-93). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.5-1 ug/ml. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.
Synonyms :
CRISP2, CRISP-2, GAPDL5, Testis-specific protein TPX-1, TPX1, TSP1, Cancer/testis antigen 36, CT36
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.