This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ B2M / Beta 2 Microglobulin Antibody (clone B2M-01, Concentrated) LS-B8113
catalog :
LS-B8113
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B2M-01

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, radioimmunoassay, immunohistochemistry - paraffin section
product information
AntibodyID :
161888
AntibodyName :
LS-B8113
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ B2M / Beta 2 Microglobulin Antibody (clone B2M-01, Concentrated) LS-B8113
Specificity :
Reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. b2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes,thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
ClonalityDesc :
Monoclonal
CloneName :
B2M-01
AntibodyModification :
Concentrated Unconjugated
PresentationDesc :
PBS containing 15 mM in sodium azide, pH 7.4
ImmunogenType :
Purified protein
ImmunogenDesc :
Purified human beta2-microglobulin
PurificationDesc :
Purified
RecommendedStorageDesc :
Store at 2-8°C.
IsotypeName :
IgG2a
Gene :
B2M / Beta 2 Microglobulin
StandardGeneSymbol :
B2M
Reactivity :
Human
NonReactiveSpecies :
Chicken, Bovine, Rabbit, Mouse, Dog
Usage :
ELISA, Flo, IHC, IHC-P (1:50), IP, RIA, WB (2 - 4 µg/ml)
ShortWebDescription :
Beta 2 Microglobulin antibody LS-B8113 is an unconjugated mouse monoclonal antibody to human Beta 2 Microglobulin (B2M). Validated for ELISA, Flow, IHC, IP, RIA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
For use in immunoprecipitation, this antibody coprecipitates b2-microglobulin (12 kDa) and HLA antigens(45 kDa) from the lysates of human lymphoid cells. Recommend U937 human histiocytic lymphoma cell line orRAJI human Burkitt lymphoma cell line as postitive control and EL4 mouse lymphoblastic lymphoma cell line as negative control for Western blot. Reducing and non-reducing conditions. Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Recommend peripheral blood lymphocytes as positve control and DAUDI human Burkitt lymphoma cell line, or erythrocytes as negative control for flow cytometry. Recommend kidney glomeruli as postive control for IHC. For RIA, use at an assay dependent concentration. The dissociation constant of the antibody soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
Synonyms :
B2M, Beta 2 Microglobulin, Beta-2-microglobulin, Beta-2-microglobin
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.