product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SFTPB / Surfactant Protein B Antibody (aa194-243) LS-B8081
catalog :
LS-B8081
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
Reference
Dillard K, Ochs M, Niskanen J, Arumilli M, Donner J, Kyöstilä K, et al. Recessive missense LAMP3 variant associated with defect in lamellar body biogenesis and fatal neonatal interstitial lung disease in dogs. PLoS Genet. 2020;16:e1008651 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B8081 image 1
Anti-Surfactant Protein B / SP-B antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 2.5-5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
161856
AntibodyName :
LS-B8081
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SFTPB / Surfactant Protein B Antibody (aa194-243) LS-B8081
Specificity :
Human SFTPB / SP-B
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa194-243
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide located between aa194-243 of human SFTPB (B2RC55, NP_000533). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Rat, Dog, Guinea pig (100%); Monkey, Mouse (92%); Marmoset, Panda, Bovine, Pig (85%); Rabbit (84%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
SFTPB / Surfactant Protein B
BlockingPeptide :
LS-E15234
StandardGeneSymbol :
SFTPB
Reactivity :
Dog, Guinea pig, Rat, Pig, Human
Usage :
IHC, IHC-P (2.5 - 5 µg/ml), WB
ShortWebDescription :
Surfactant Protein B antibody LS-B8081 is an unconjugated rabbit polyclonal antibody to Surfactant Protein B (SFTPB) (aa194-243) from human. It is reactive with human, rat, dog and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Synonyms :
SFTPB, 6 kDa protein, PSP-B, SFTP3, SP-B, SFTB3, SMDP1, Surfactant protein B
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.