product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ TP53 / p53 Antibody (aa283-332) LS-B7722
catalog :
LS-B7722
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse
  • western blot; mouse
Malchenko S, Sredni S, Bi Y, Margaryan N, Boyineni J, Mohanam I, et al. Stabilization of HIF-1α and HIF-2α, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. PLoS ONE. 2017;12:e0173106 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B7722 image 1
Anti-p53 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
image 2 :
LifeSpan Biosciences LS-B7722 image 2
Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.
image 3 :
LifeSpan Biosciences LS-B7722 image 3
Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using p53 Antibody. The lane on the right is blocked with the synthesized peptide.
product information
AntibodyID :
159086
AntibodyName :
LS-B7722
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
PathPlus™ TP53 / p53 Antibody (aa283-332) LS-B7722
Specificity :
This antibody detects endogenous levels of total p53 protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa283-332
PresentationDesc :
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
ImmunogenDesc :
Synthetic peptide from human p53. (AA Range: 283-332)
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C for up to 1 year.
IsotypeName :
IgG
Gene :
TP53 / p53
StandardGeneSymbol :
TP53
gene family :
P53
Reactivity :
Mouse, Rat, Human
Usage :
IF (1:100 - 1:500), IHC, IHC-P (1:100), Peptide-ELISA (1:10000), WB (1:500 - 1:1000)
ShortWebDescription :
P53 antibody LS-B7722 is an unconjugated rabbit polyclonal antibody to p53 (TP53) (aa283-332) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Cited in 1 publication.
UsageText :
Western Blot: Predicted MW 43 kDa
Synonyms :
TP53, BCC7, Cellular tumor antigen p53, p53, TRP53, p53 tumor suppressor, Phosphoprotein p53, Antigen NY-CO-13, Tumor protein p53, Tumor suppressor p53, LFS1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.