This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Sprouty 4 / SPRY4 Antibody (aa275-299) LS-B77
catalog :
LS-B77
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
27117
AntibodyName :
LS-B77
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ Sprouty 4 / SPRY4 Antibody (aa275-299) LS-B77
Specificity :
A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa275-299
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
IsotypeName :
IgG
Gene :
Sprouty 4 / SPRY4
StandardGeneSymbol :
SPRY4
Reactivity :
Human, Chimpanzee
Usage :
ELISA (1:3000 - 1:12000), IHC, IHC-P (2.5 µg/ml), WB (1:100 - 1:1000)
ShortWebDescription :
SPRY4 antibody LS-B77 is an unconjugated rabbit polyclonal antibody to SPRY4 (Sprouty 4) (aa275-299) from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B77 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B77 was determined to be 2.5 ug/ml.
Synonyms :
SPRY4, Protein sprouty homolog 4, Sprouty 4, Sprouty homolog 4 (Drosophila), Sprouty 4, Spry-4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.