This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ GLI2 Antibody (Internal) LS-B75
catalog :
LS-B75
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
27115
AntibodyName :
LS-B75
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
IHC-plus™ GLI2 Antibody (Internal) LS-B75
Specificity :
A BLAST analysis was used to suggest cross-reactivity with Gli-2 from mouse and rat sources based on 100% sequence homology with the immunogen. Reactivity with Gli-2 from other sources is not known.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
Internal
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
GLI2
StandardGeneSymbol :
GLI2
Reactivity :
Mouse
Usage :
ELISA (1:15000 - 1:60000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000)
ShortWebDescription :
GLI2 antibody LS-B75 is an unconjugated rabbit polyclonal antibody to mouse GLI2 (Internal). Validated for ELISA, IHC and WB.
UsageText :
Immunohistochemistry: LS-B75 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B75 was determined to be 5 ug/ml.
Synonyms :
GLI2, GLI-Kruppel family member GLI2, HPE9, Gli-2, Oncogene GLI2, Tax helper protein 2, THP, Zinc finger protein GLI2, Tax helper protein, THP2, GLI family zinc finger 2, Tax helper protein 1, THP1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.