This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ TIMP1 Antibody (aa24-207) LS-B7485
catalog :
LS-B7485
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
156915
AntibodyName :
LS-B7485
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ TIMP1 Antibody (aa24-207) LS-B7485
Specificity :
Human TIMP-1
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa24-207
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenDesc :
E.coli derived recombinant Human TIMP-1 encoding aa 24-207
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute with sterile water
IsotypeName :
IgG
Gene :
TIMP1
StandardGeneSymbol :
TIMP1
Reactivity :
Human
Usage :
ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
TIMP1 antibody LS-B7485 is an unconjugated rabbit polyclonal antibody to human TIMP1 (aa24-207). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B7485 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human TIMP-1 (3 ug/ml), a concentration of 5 ug/ml of this antibody is required. ELISA: To detect hTIMP-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TIMP-1 (LS-C104724) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTIMP-1. Western Blot: To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
TIMP1, CLGI, EPA, HCI, Metalloproteinase inhibitor 1, TIMP-1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.