product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ IL6 / Interleukin 6 Antibody LS-B7482
catalog :
LS-B7482
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry - paraffin section
citations: 1
Reference
Boonkasidecha S, Kannan P, Kallapur S, Jobe A, Kemp M. Fetal skin as a pro-inflammatory organ: Evidence from a primate model of chorioamnionitis. PLoS ONE. 2017;12:e0184938 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B7482 image 1
Anti-IL-6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
156912
AntibodyName :
LS-B7482
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
PathPlus™ IL6 / Interleukin 6 Antibody LS-B7482
Specificity :
Human IL-6
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
Lyophilized from PBS, pH 7.2
ImmunogenType :
Cell extract
ImmunogenDesc :
E.coli derived recombinant Human IL-6.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ResuspensionLiquidDesc :
Reconstitute in sterile water to 0.1-1 mg/ml.
IsotypeName :
IgG
Gene :
IL6 / Interleukin 6
StandardGeneSymbol :
IL6
gene family :
Hormone
Reactivity :
Human
Usage :
ELISA (0.5 - 2 µg/ml), IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
ShortWebDescription :
Interleukin 6 antibody LS-B7482 is an unconjugated rabbit polyclonal antibody to human Interleukin 6 (IL6). Validated for ELISA, IHC and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B7482 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-6 (1 ng/ml), a concentration of 0.20-0.40 ug/ml of this antibody is required. ELISA: To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 (LS-C104546) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Synonyms :
IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.