product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ATP1B1 Antibody (aa85-99) LS-B7227
catalog :
LS-B7227
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:2000
Shah V, Meyerholz D, Tang X, Reznikov L, Abou Alaiwa M, Ernst S, et al. Airway acidification initiates host defense abnormalities in cystic fibrosis mice. Science. 2016;351:503-7 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B7227 image 1
ATP1B1 antibody (0.1 ug/ml) staining of Rat Retina lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
image 2 :
LifeSpan Biosciences LS-B7227 image 2
Anti-ATP1B1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
155618
AntibodyName :
LS-B7227
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ ATP1B1 Antibody (aa85-99) LS-B7227
Specificity :
Human ATP1B1. This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa85-99
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-KTEISFRPNDPKSYE, from the internal region of the protein sequence according to NP_001668.1NP_001001787.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
ATP1B1
BlockingPeptide :
LS-E27172
StandardGeneSymbol :
ATP1B1
gene family :
Transporter
Subfamily :
ATPase - P type, beta subunit
Reactivity :
Mouse, Dog, Rat, Hamster, Horse, Human
Usage :
IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.1 - 0.3 µg/ml)
ShortWebDescription :
ATP1B1 antibody LS-B7227 is an unconjugated goat polyclonal antibody to ATP1B1 (aa85-99) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kDa band observed in Rat Retina lysates and 50kDa in Mouse and Rat Kidney lysates (calculated MW of 35.1kDa according to Human NP_001668.1, 35.2kDa according to Mouse NP_033851.1 and Rat NP_037245.2).The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 ug/ml.
Synonyms :
ATP1B1, Adenosinetriphosphatase, ATP1B, Atpb1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.