This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NES / Nestin Antibody LS-B656
catalog :
LS-B656
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
32884
AntibodyName :
LS-B656
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ NES / Nestin Antibody LS-B656
Specificity :
A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against homologues from other sources is not known.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
IsotypeName :
IgG
Gene :
NES / Nestin
StandardGeneSymbol :
NES
Reactivity :
Mouse, Human
Usage :
ELISA (1:20000 - 1:80000), IHC (5 µg/ml), IHC-P (5 µg/ml), WB (1:500 - 1:3000)
ShortWebDescription :
Nestin antibody LS-B656 is an unconjugated rabbit polyclonal antibody to Nestin (NES) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B656 was determined to be 5 ug/ml.
Synonyms :
NES, Nestin, Nbla00170
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.