product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ RELA / NFKB p65 Antibody (phospho-Ser529) LS-B652
catalog :
LS-B652
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Su E, Zhao L, Yang X, Zhu B, Liu Y, Zhao W, et al. Aggravated endothelial endocrine dysfunction and intimal thickening of renal artery in high-fat diet-induced obese pigs following renal denervation. BMC Cardiovasc Disord. 2020;20:176 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B652 image 1
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
product information
AntibodyID :
32880
AntibodyName :
LS-B652
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ RELA / NFKB p65 Antibody (phospho-Ser529) LS-B652
Specificity :
This phospho specific polyclonal antibody is specific for phosphorylated pS529 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS529 phosphorylated p65 from mouse, rat or other species has not been determined.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pSer529
PresentationDesc :
Antiserum, 0.1% sodium azide
ImmunogenType :
Native protein
ImmunogenDesc :
NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
PurificationDesc :
Delipidated and defibrinated
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Gene :
RELA / NFKB p65
StandardGeneSymbol :
RELA
Reactivity :
Human
Usage :
ELISA (1:5000 - 1:25000), IHC, IHC-P (1:500), WB (1:500 - 1:3000)
ShortWebDescription :
NFKB p65 antibody LS-B652 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA) (pSer529). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 5 publications.
UsageText :
Immunohistochemistry: LS-B652 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B652 was determined to be 1:500.
Synonyms :
RELA, NF-kappa-B p65delta3, NF-kappaB, NFKB3, p65, Transcription factor p65, NF kappaB, NFKB
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.