IHC-plus™ PPARG / PPAR Gamma Antibody LS-B651 | LifeSpan Biosciences LS-B651 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ PPARG / PPAR Gamma Antibody LS-B651

catalog :

LS-B651

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, dogs

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
flow cytometry; dogs immunocytochemistry; dogs western blot; dogs; 1:500; fig 8	Duan W, Lopez M. Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue. Stem Cell Rev. 2016;12:257-68 pubmed publisher
	Yoo K, Kwon T, Oh C, Ko K, No Y, Oh W, et al. Improvement of a slimming cream's efficacy using a novel fabric as a transdermal drug delivery system: An in vivo and in vitro study. Exp Ther Med. 2020;19:3282-3288 pubmed publisher

image

image 1 :

Anti-PPARG antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

AntibodyID :

32879

AntibodyName :

LS-B651

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

IHC-plus™ PPARG / PPAR Gamma Antibody LS-B651

Specificity :

A BLAST analysis was used to suggest broad reactivity with this protein from several sources. Expect reactivity against human (g1 and g2), mouse (g1 and g2), rat, squirrel, orangutan, duck (g1), macaque (g1-g7), boar (g1a-1d), mink, guinea pig, rabbit (g1 and g3), dog and hamster sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR gamma homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

PresentationDesc :

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-300 of human PPAR gamma isoform 1.

PurificationDesc :

Affinity purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

IsotypeName :

IgG

Gene :

PPARG / PPAR Gamma

StandardGeneSymbol :

PPARG

gene family :

NHR

Subfamily :

NR1 Thyroid hormone-like

Reactivity :

Duck, Squirrel, Boar, Rabbit, Mouse, Dog, Guinea pig, Rat, Hamster, Mink, Macaque, Orangutan, Human

Usage :

ELISA (1:8000 - 1:32000), IHC, IHC-P (5 µg/ml), WB

ShortWebDescription :

PPAR Gamma antibody LS-B651 is an unconjugated rabbit polyclonal antibody to PPAR Gamma (PPARG) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.

UsageText :

Immunohistochemistry: LS-B651 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B651 was determined to be 5 ug/ml.

Synonyms :

PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage