This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MKI67 / Ki67 Antibody (clone Ki-S5) LS-B6433
catalog :
LS-B6433
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Ki-S5
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
product information
AntibodyID :
151884
AntibodyName :
LS-B6433
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ MKI67 / Ki67 Antibody (clone Ki-S5) LS-B6433
Specificity :
Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Confined to the nuclei of proliferating cells and no cross-reactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
ClonalityDesc :
Monoclonal
CloneName :
Ki-S5
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.6, 0.09% Sodium Azide
ImmunogenType :
Cells
ImmunogenDesc :
Nuclear protein preparation from the human cell line U937.50.
PurificationDesc :
Protein A purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
MKI67 / Ki67
StandardGeneSymbol :
MKI67
Reactivity :
Human
Usage :
Flo, ICC (5 - 10 µg/ml), IHC, IHC-P (10 µg/ml), WB (1:500)
ShortWebDescription :
Ki67 antibody LS-B6433 is an unconjugated mouse monoclonal antibody to human Ki67 (MKI67). Validated for Flow, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Western Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10 ug of A431 lysates. Immunocytochemistry: 5-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Positive control: Tonsil tissue, A431 Cell Lysate.
Synonyms :
MKI67, Antigen KI-67, Ki67, KIA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.