IHC-plus™ SMAD3 Antibody (phospho-Ser423/425) LS-B64 | LifeSpan Biosciences LS-B64 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ SMAD3 Antibody (phospho-Ser423/425) LS-B64

catalog :

LS-B64

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Deng Z, Gao X, Sun X, Amra S, Lu A, Cui Y, et al. Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice. FASEB J. 2019;33:8809-8821 pubmed publisher

image

image 1 :

Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

AntibodyID :

27104

AntibodyName :

LS-B64

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

IHC-plus™ SMAD3 Antibody (phospho-Ser423/425) LS-B64

Specificity :

Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

pSer423/425

PresentationDesc :

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein.

PurificationDesc :

Affinity purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

IsotypeName :

IgG

Gene :

SMAD3

StandardGeneSymbol :

SMAD3

Reactivity :

Xenopus laevis, Xenopus tropicalis, Chicken, Zebrafish, Pig, Bovine, Mouse, Rat, Human

Usage :

ELISA (1:15000 - 1:30000), Flo, IHC, IHC-P (2.5 µg/ml), WB (1:2000 - 1:20000)

ShortWebDescription :

SMAD3 antibody LS-B64 is an unconjugated rabbit polyclonal antibody to SMAD3 (pSer423/425) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B64 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B64 was determined to be 2.5 ug/ml.

Synonyms :

SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage