product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ANGPT1 / Angiopoietin-1 Antibody (aa15-45) LS-B62
catalog :
LS-B62
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 1
Reference
de Vries M, Parma L, Peters H, Schepers A, Hamming J, Jukema J, et al. Blockade of vascular endothelial growth factor receptor 2 inhibits intraplaque haemorrhage by normalization of plaque neovessels. J Intern Med. 2019;285:59-74 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B62 image 1
Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of human lung, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
product information
AntibodyID :
27102
AntibodyName :
LS-B62
TargetSpecies :
Mouse
Host Species :
Rabbit
Product Name :
IHC-plus™ ANGPT1 / Angiopoietin-1 Antibody (aa15-45) LS-B62
Specificity :
This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa15-45
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
PurificationDesc :
Delipidated and defibrinated
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Gene :
ANGPT1 / Angiopoietin-1
StandardGeneSymbol :
ANGPT1
Reactivity :
Mouse, Human
Usage :
ELISA (1:5000 - 1:25000), IHC, IHC-P (1:200), IP, WB (1:500 - 1:2000)
ShortWebDescription :
Angiopoietin-1 antibody LS-B62 is an unconjugated rabbit polyclonal antibody to Angiopoietin-1 (ANGPT1) (aa15-45) from mouse. It is reactive with human and mouse. Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B62 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B62 was determined to be 1:200.
Synonyms :
ANGPT1, Angiopoietin 1, Angiopoietin-1, AGPT, AGP1, ANG-1, ANG1, KIAA0003
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.