product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CES2 / Esterase Antibody (clone 4F12) LS-B6190
catalog :
LS-B6190
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4F12
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human
Verbrugge S, Al M, Assaraf Y, Kammerer S, Chandrupatla D, Honeywell R, et al. Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells: down-regulation of carboxylesterase 1, drug sequestration in lipid droplets and pro-survival activation ERK/Akt/mTOR. Oncotarget. 2016;7:5240-57 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B6190 image 1
Western blot of CES2 expression in HepG2.
image 2 :
LifeSpan Biosciences LS-B6190 image 2
Detection limit for recombinant GST tagged CES2 is approximately 0.3 ng/ml as a capture antibody.
image 3 :
LifeSpan Biosciences LS-B6190 image 3
Anti-CES2 / Esterase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
149144
AntibodyName :
LS-B6190
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ CES2 / Esterase Antibody (clone 4F12) LS-B6190
Specificity :
Human CES2
ClonalityDesc :
Monoclonal
CloneName :
4F12
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
CES2 (NP_003860, 514 aa ~ 622 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
PurificationDesc :
Purified from ascites by Protein A
RecommendedStorageDesc :
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
IsotypeName :
IgG2b,k
Gene :
CES2 / Esterase
StandardGeneSymbol :
CES2
Reactivity :
Human
Usage :
ELISA, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
ShortWebDescription :
Esterase antibody LS-B6190 is an unconjugated mouse monoclonal antibody to human Esterase (CES2). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using the recombinant protein used as the immunogen.
Synonyms :
CES2, Carboxylesterase 2, CE-2, CES2A1, Cocaine esterase, Esterase, ICE, HCE-2, PCE-2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.