product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SP1 Antibody (clone 1A5) LS-B6148
catalog :
LS-B6148
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1A5
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Chhunchha B, Kubo E, Singh P, Singh D. Sumoylation-deficient Prdx6 repairs aberrant Sumoylation-mediated Sp1 dysregulation-dependent Prdx6 repression and cell injury in aging and oxidative stress. Aging (Albany NY). 2018;10:2284-2315 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B6148 image 1
SP1 monoclonal antibody (M02), clone 1A5 Western blot of SP1 expression in HeLa NE.
image 2 :
LifeSpan Biosciences LS-B6148 image 2
Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml]
image 3 :
LifeSpan Biosciences LS-B6148 image 3
Detection limit for recombinant GST tagged SP1 is approximately 0.1 ng/ml as a capture antibody.
product information
AntibodyID :
148421
AntibodyName :
LS-B6148
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ SP1 Antibody (clone 1A5) LS-B6148
Specificity :
Human SP1
ClonalityDesc :
Monoclonal
CloneName :
1A5
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
SP1 (NP_612482, 89 aa ~ 199 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
PurificationDesc :
Purified from ascites by Protein A
RecommendedStorageDesc :
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
SP1
StandardGeneSymbol :
SP1
gene family :
Transcription factor
Reactivity :
Mouse, Human
Usage :
ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
ShortWebDescription :
SP1 antibody LS-B6148 is an unconjugated mouse monoclonal antibody to SP1 from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Recombinant protein.
Synonyms :
SP1, Sp1 transcription factor, Transcription factor Sp1, Specificity protein 1, TSFP1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.