product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ Peripherin Antibody (aa374-471, clone 3B3) LS-B6138
catalog :
LS-B6138
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3B3
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
citations: 2
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:400; fig 3a
Johnson Chacko L, Pechriggl E, Fritsch H, Rask Andersen H, Blumer M, Schrott Fischer A, et al. Neurosensory Differentiation and Innervation Patterning in the Human Fetal Vestibular End Organs between the Gestational Weeks 8-12. Front Neuroanat. 2016;10:111 pubmed
  • immunohistochemistry - paraffin section; human; 1:400
Pechriggl E, Bitsche M, Glueckert R, Rask Andersen H, Blumer M, Schrott Fischer A, et al. Development of the innervation of the human inner ear. Dev Neurobiol. 2015;75:683-702 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B6138 image 1
Western blot of PRPH expression in IMR-32.
image 2 :
LifeSpan Biosciences LS-B6138 image 2
Detection limit for recombinant GST tagged PRPH is 1 ng/ml as a capture antibody.
image 3 :
LifeSpan Biosciences LS-B6138 image 3
Anti-PRPH / Peripherin antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
148387
AntibodyName :
LS-B6138
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PathPlus™ Peripherin Antibody (aa374-471, clone 3B3) LS-B6138
Specificity :
Human Peripherin
ClonalityDesc :
Monoclonal
CloneName :
3B3
AntibodyModification :
Unconjugated
AntigenModification :
aa374-471
PresentationDesc :
PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
PRPH (NP_006253, 374 aa ~ 471 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
PurificationDesc :
Purified from ascites by Protein A
RecommendedStorageDesc :
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
IsotypeName :
IgG2b,k
Gene :
Peripherin
StandardGeneSymbol :
PRPH
Reactivity :
Human
Usage :
ELISA, IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
ShortWebDescription :
Peripherin antibody LS-B6138 is an unconjugated mouse monoclonal antibody to human Peripherin (aa374-471). Validated for ELISA, IHC and WB.
UsageText :
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates and the recombinant protein used as the immunogen.
Synonyms :
PRPH, NEF4, Neurofilament 4 (57kD), Peripherin, PRPH1, Neurofilament 4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.