product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ EP300 / p300 Antibody (clone 1D2) LS-B6081
catalog :
LS-B6081
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1D2
reactivity :
human
application :
western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Dong L, You S, Zhang Q, Osuka S, Devi N, Kaluz S, et al. Arylsulfonamide 64B Inhibits Hypoxia/HIF-Induced Expression of c-Met and CXCR4 and Reduces Primary Tumor Growth and Metastasis of Uveal Melanoma. Clin Cancer Res. 2019;25:2206-2218 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B6081 image 1
Western blot of EP300 expression in HeLa nuclear extract.
image 2 :
LifeSpan Biosciences LS-B6081 image 2
Immunofluorescence of monoclonal antibody to EP300 on HeLa cell. [antibody concentration 10 ug/ml]
image 3 :
LifeSpan Biosciences LS-B6081 image 3
Detection limit for recombinant GST tagged EP300 is approximately 0.03 ng/ml as a capture antibody.
product information
AntibodyID :
144980
AntibodyName :
LS-B6081
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ EP300 / p300 Antibody (clone 1D2) LS-B6081
Specificity :
Human p300
ClonalityDesc :
Monoclonal
CloneName :
1D2
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.4
ImmunogenType :
Recombinant protein
ImmunogenDesc :
EP300 (NP_001420, 731 aa ~ 831 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
PurificationDesc :
Purified from ascites by Protein A
RecommendedStorageDesc :
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
EP300 / p300
StandardGeneSymbol :
EP300
Reactivity :
Human
Usage :
ELISA, IF (10 µg/ml), IHC-P (5 µg/ml), WB (1:500 - 1:1000)
ShortWebDescription :
P300 antibody LS-B6081 is an unconjugated mouse monoclonal antibody to human p300 (EP300). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Synonyms :
EP300, E1A binding protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT, E1A-associated protein p300, E1A-binding protein, 300kD, p300, RSTS2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.