product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Mcpt4 Antibody (aa114-126) LS-B5958
catalog :
LS-B5958
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
mouse
application :
immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Chapman N, Zeng H, Nguyen T, Wang Y, Vogel P, Dhungana Y, et al. mTOR coordinates transcriptional programs and mitochondrial metabolism of activated Treg subsets to protect tissue homeostasis. Nat Commun. 2018;9:2095 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5958 image 1
Anti-MCPT4 antibody IHC of mouse skeletal muscle, mast cell. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
product information
AntibodyID :
143631
AntibodyName :
LS-B5958
TargetSpecies :
Mouse
Host Species :
Goat
Product Name :
IHC-plus™ Mcpt4 Antibody (aa114-126) LS-B5958
Specificity :
Mouse MCPT4.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa114-126
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-KLQKKAKETPSVN, from the internal region of the protein sequence according to NP_034909.2.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
Mcpt4
BlockingPeptide :
LS-E26994
StandardGeneSymbol :
Mcpt4
gene family :
Protease
Subfamily :
Serine S1
Reactivity :
Mouse
Usage :
IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:64000)
ShortWebDescription :
Mcpt4 antibody LS-B5958 is an unconjugated goat polyclonal antibody to mouse Mcpt4 (aa114-126). Validated for IHC and Peptide-ELISA. Cited in 1 publication.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave bands at approx. 40kDa and 15kDa in Mouse Skeletal Muscle lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 27.2kDa according to NP_034909.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Synonyms :
Mcpt4, MCP-4, Mast cell protease 4, MCP4, Mmcp4, MMCP-4A, MMCP-4B, MMCP-4
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.