product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ITGAL / CD11a Antibody (aa953-965) LS-B5915
catalog :
LS-B5915
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
Reference
Figenschau S, Knutsen E, Urbarova I, Fenton C, Elston B, Perander M, et al. ICAM1 expression is induced by proinflammatory cytokines and associated with TLS formation in aggressive breast cancer subtypes. Sci Rep. 2018;8:11720 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5915 image 1
ITGAL / CD11a antibody (0.5µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
image 2 :
LifeSpan Biosciences LS-B5915 image 2
Anti-ITGAL / CD11a antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
product information
AntibodyID :
141322
AntibodyName :
LS-B5915
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ ITGAL / CD11a Antibody (aa953-965) LS-B5915
Specificity :
Human ITGAL / CD11a. This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa953-965
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-YQVRIQPSIHDHN, from the internal region of the protein sequence according to NP_002200.2NP_001107852.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
ITGAL / CD11a
BlockingPeptide :
LS-E27116
StandardGeneSymbol :
ITGAL
gene family :
Integrin
Reactivity :
Human, Monkey
Usage :
IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.5 - 2 µg/ml)
ShortWebDescription :
CD11a antibody LS-B5915 is an unconjugated goat polyclonal antibody to CD11a (ITGAL) (aa953-965) from human. It is reactive with human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2 ug/ml.
Synonyms :
ITGAL, CD11A, Integrin alpha-L, LFA-1 alpha, LFA-1A, Integrin gene promoter, Alpha polypeptide), CD11a antigen, LFA-1, LFA1A
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.