product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ARF6 Antibody (C-Terminus) LS-B5846
catalog :
LS-B5846
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
Reference
Liu Y, Yu M, Shang X, Nguyen M, Balakrishnan S, Sager R, et al. Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors. Sci Rep. 2020;10:7795 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5846 image 1
Anti-ARF6 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
141253
AntibodyName :
LS-B5846
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ ARF6 Antibody (C-Terminus) LS-B5846
Specificity :
Human ARF6
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from C-Terminus of human ARF6 (P62330, NP_001654). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Catfish, Salmon, Smelt, Zebrafish, Drosophila, Beetle (100%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
ARF6
BlockingPeptide :
LS-E19666
StandardGeneSymbol :
ARF6
gene family :
Ras GTPase superfamily IPR001806
Reactivity :
Xenopus, Chicken, Zebrafish, Drosophila, Pig, Horse, Bat, Rat, Hamster, Sheep, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
ShortWebDescription :
ARF6 antibody LS-B5846 is an unconjugated rabbit polyclonal antibody to ARF6 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Synonyms :
ARF6, ADP-ribosylation factor 6
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.