product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ LEFTYB / LEFTY1 Antibody (N-Terminus) LS-B5830
catalog :
LS-B5830
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig s14
Hughes C, McConechy M, Cochrane D, Nazeran T, Karnezis A, Huntsman D, et al. Quantitative Profiling of Single Formalin Fixed Tumour Sections: proteomics for translational research. Sci Rep. 2016;6:34949 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5830 image 1
Anti-LEFTY1 / Lefty antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
141237
AntibodyName :
LS-B5830
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ LEFTYB / LEFTY1 Antibody (N-Terminus) LS-B5830
Specificity :
Human LEFTY1 / Lefty
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from N-Terminus of human LEFTY1 (O75610, NP_066277). Percent identity by BLAST analysis: Human (100%); Gorilla (92%); Gibbon, Marmoset (85%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Gene :
LEFTYB / LEFTY1
BlockingPeptide :
LS-E18838
StandardGeneSymbol :
LEFTY1
gene family :
TGF beta
Reactivity :
Human
Usage :
IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
ShortWebDescription :
LEFTY1 antibody LS-B5830 is an unconjugated rabbit polyclonal antibody to human LEFTY1 (LEFTYB) (N-Terminus). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Synonyms :
LEFTY1, LEFTYB, Protein lefty-B, LEFTB, Protein lefty-1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.