product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ABCB4 / MDR3 Antibody (aa252-301) LS-B5729
catalog :
LS-B5729
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; rat; loading ...; fig 5a
Zhao G, Xu D, Yuan Z, Jiang Z, Zhou W, Li Z, et al. 8-Methoxypsoralen disrupts MDR3-mediated phospholipids efflux and bile acid homeostasis and its relevance to hepatotoxicity. Toxicology. 2017;386:40-48 pubmed publisher
Weber N, Odriozola L, Martínez García J, Ferrer V, Douar A, Bénichou B, et al. Gene therapy for progressive familial intrahepatic cholestasis type 3 in a clinically relevant mouse model. Nat Commun. 2019;10:5694 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5729 image 1
Anti-ABCB4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
140335
AntibodyName :
LS-B5729
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ ABCB4 / MDR3 Antibody (aa252-301) LS-B5729
Specificity :
Human ABCB4
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa252-301
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide located between aa252-301 of human ABCB4 (A4D1D4, NP_000434). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit (100%); Galago, Panda, Opossum (92%); Bovine (85%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Gene :
ABCB4 / MDR3
BlockingPeptide :
LS-E15357
StandardGeneSymbol :
ABCB4
gene family :
Transporter
Subfamily :
ATP-binding cassette - ABCB/MDR
Reactivity :
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
ShortWebDescription :
MDR3 antibody LS-B5729 is an unconjugated rabbit polyclonal antibody to MDR3 (ABCB4) (aa252-301) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Synonyms :
ABCB4, ABC21, ICP3, GBD1, MDR3, MDR2, Multidrug resistance protein 3, Multiple drug resistance 3, P-glycoprotein 3, Pgy2, PGY3, PFIC-3, MDR2/3
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.