product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ FZD4 / Frizzled 4 Antibody (C-Terminus) LS-B5723
catalog :
LS-B5723
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Kolbe E, Aleithe S, Rennert C, Spormann L, Ott F, Meierhofer D, et al. Mutual Zonated Interactions of Wnt and Hh Signaling Are Orchestrating the Metabolism of the Adult Liver in Mice and Human. Cell Rep. 2019;29:4553-4567.e7 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5723 image 1
Anti-FZD4 / Frizzled 4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
product information
AntibodyID :
140329
AntibodyName :
LS-B5723
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ FZD4 / Frizzled 4 Antibody (C-Terminus) LS-B5723
Specificity :
Human FZD4 / Frizzled 4
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
C-Terminus
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from C-Terminus of human FZD4 (Q9ULV1, NP_036325). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%); Platypus, Xenopus (92%); Lizard (86%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
FZD4 / Frizzled 4
BlockingPeptide :
LS-E15169
StandardGeneSymbol :
FZD4
gene family :
GPCR
Subfamily :
Frizzled
Reactivity :
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Pig, Horse, Bat, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
ShortWebDescription :
Frizzled 4 antibody LS-B5723 is an unconjugated rabbit polyclonal antibody to Frizzled 4 (FZD4) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Synonyms :
FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.