This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ABCB1 / MDR1 / P Glycoprotein Antibody (clone JSB-1) LS-B5570
catalog :
LS-B5570
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
JSB-1

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
140176
AntibodyName :
LS-B5570
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ ABCB1 / MDR1 / P Glycoprotein Antibody (clone JSB-1) LS-B5570
Specificity :
The antibody JSB-1 reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170-180 kD glycoprotein, the expression of which is strongly correlated with the degree of multi-drug-resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas. Cross-reacts with Chinese hamster. No cross-reactivity with mouse or rat.
ClonalityDesc :
Monoclonal
CloneName :
JSB-1
AntibodyModification :
Unconjugated
PresentationDesc :
Tissue Culture Supernatant, 0.1% Sodium Azide, 1% BSA
ImmunogenType :
Recombinant protein
PurificationDesc :
Tissue culture supernatant
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
ABCB1 / MDR1 / P Glycoprotein
StandardGeneSymbol :
ABCB1
gene family :
Transporter
Subfamily :
ATP-binding cassette - ABCB/MDR
Reactivity :
Human
Usage :
Flo, IHC, IHC-Fr, IHC-P (1:50), WB
ShortWebDescription :
P Glycoprotein antibody LS-B5570 is an unconjugated mouse monoclonal antibody to human P Glycoprotein (ABCB1 / MDR1). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
JSB-1 has potential value for diagnostic detection of MDR cells in human tumor samples. Working dilutions. Flow cytometry: at least 1:10. Western blotting: start optimizing working dilution a 1:10. Immunocytochemistry: acetone fixed cell preparation at least 1:20. Immunohistochemistry: acetone-fixed frozen sections at least 1:20, formalin-fixed paraffin embedded tissues 1:20. Instructions for use Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used (see below) also paraffin embedded tissue can be stained with the antibody. In Flow cytometry fixate cells in 10% (v/v) Lysing solution followed by primary antibody and antiMouse-FITC. In developing a Western blot staining pattern use an anti-Mouse-HRP.
Synonyms :
ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.