antibody

IHC-plus™ NCAM / CD56 Antibody (clone 123C3, Concentrated) LS-B5568

LS-B5568

monoclonal

mouse

nonconjugated

123C3

immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

LS-B5568

Human

Mouse

IHC-plus™ NCAM / CD56 Antibody (clone 123C3, Concentrated) LS-B5568

The antibody recognizes a transmembrane glycoprotein of 140 and 180 kD which has been identified as NCAM (Neural Cell Adhesion Module). It is involved in intercellular adhesion and play a role in outgrowth of neurites and the development of the nervous system. Several other isoforms have been identified which are expressed in a developmental and tissue specific pattern. Two major epitopes have been defined, NKI-nbl-1 (MON 9012) reacts with epitope 1 and 123C3 (MON 9006) reacts with epitope 2. At the international Workshop on SCLC antibodies 123C3 has been categorized as cluster 1 antibody (Stahel et al 1994). Most importantly, 123C3 can be used on routing formalin fixed paraffin section after retrieval. All cells in small cell carcinomas and carcinoids of the lung are strongly positive for 123C3. A minority of cases of other major types of lung carcinoma are sometimes positive as well: however this positivity is generally weak and focal. Adenoid cystic carcinomas of bronchial glands are strongly positive. Neuroblastoma's and Wilms tumors are usually also staining strongly positive. In non small lung cell carcinomas, 123C3 staining has been associated with more advanced stage and a decreased survival after surgery (Kibbelaar et al. 1991). Positive staining with other tumors, include medullary thyroid carcinomas and some ovarian tumors. NCAM (CD56) is a marker for natural killer: 123C3 can be used to distinguish NK cells from other hematopoietic cell populations. Although .

Monoclonal

123C3

Concentrated Unconjugated

Tissue Culture Supernatant, 0.02% Sodium Azide

Native protein

Tissue culture supernatant

Store at 2-8°C.

IgG1

NCAM / CD56

NCAM1

Immunoglobulin

Human

IF, IHC, IHC-Fr, IHC-P (1:50)

CD56 antibody LS-B5568 is an unconjugated mouse monoclonal antibody to human CD56 (NCAM ). Validated for IF and IHC. Tested on 20 paraffin-embedded human tissues.

Study of small cell carcinoma. Detection of CD56 positive lymphomas. The antibody is useful on formalin fixed paraffin sections. These sections should be pretreated in microwave or pressure cooker. An optimal dilution should be determined at each individual laboratory. For use in staining of frozen sections see instructions I. For regular immunoperoxidase and immunofluorescence tests a 1:5 to 1:20 dilution in PBS is advised.

NCAM1, CD56, CD56 antigen, MSK39, NCAM-1, N-CAM-1, NCAM

Worldwide