This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ Endothelium Antibody (clone EN4, Concentrated) LS-B5561
catalog :
LS-B5561
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
EN4
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
140167
AntibodyName :
LS-B5561
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ Endothelium Antibody (clone EN4, Concentrated) LS-B5561
Specificity :
This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.
ClonalityDesc :
Monoclonal
CloneName :
EN4
AntibodyModification :
Concentrated Unconjugated
PresentationDesc :
PBS, 0.02% Sodium Azide, 0.1% BSA
ImmunogenType :
Tissue
ImmunogenDesc :
Endothelium
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Store at 2-8°C.
IsotypeName :
IgG1
Gene :
Endothelium
Reactivity :
Human
Usage :
Flo, IHC, IHC-Fr, IHC-P (1:20 - 1:100)
ShortWebDescription :
Endothelium antibody LS-B5561 is an unconjugated mouse monoclonal antibody to human Endothelium. Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
The antibody is useful for immunohistological staining of endothelia in frozen sections. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained. The antibody works well on acetone fixed or unfixed cryostat sections, and periodate/lysine/ paraformaldehyde-fixed sections, in which membrane antigens and the structures of vessels and adjacent tissues are optimally preserved.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.