This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CD34 Antibody (clone QBEND-10, Concentrated) LS-B5552
catalog :
LS-B5552
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
QBEND-10

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
140158
AntibodyName :
LS-B5552
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ CD34 Antibody (clone QBEND-10, Concentrated) LS-B5552
Specificity :
The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on hematopoietic cells and on the established myeloid leukemic cell line KG1A. QBEND-10 recognizes the Class II epitope on CD34. This antibody does not cross-react with tested species such as sheep, cow, dog and rat. Clone is reported to cross react with Rhesus Monkey (2). Immunogen: human endothelial vesicles.
ClonalityDesc :
Monoclonal
CloneName :
QBEND-10
AntibodyModification :
Concentrated Unconjugated
PresentationDesc :
PBS, 0.09% Sodium Azide
ImmunogenType :
Membrane preparation
ImmunogenDesc :
Human endothelial vesicles.
PurificationDesc :
Purified
RecommendedStorageDesc :
Store at 2-8°C.
IsotypeName :
IgG1
Gene :
CD34
StandardGeneSymbol :
CD34
Reactivity :
Human
Usage :
Flo, IHC, IHC-Fr, IHC-P (1:800), IP, WB
ShortWebDescription :
CD34 antibody LS-B5552 is an unconjugated mouse monoclonal antibody to human CD34. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
The antibody can be used for flow cytometry, immunohistochemistry on frozen and paraffin embedded section, immunoprecipitation and Western blotting. Instructions for use Flow cytometry: Use 10 ul antibody (1:100-1:200 dilution) to label 106 cells in 100 ul Immunohistochemistry: This product does not require antigen retrieval using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Recommended dilution: 1:500 - 1:1000 for immunohistochemistry on paraffin sections. Recommended positive control tissue: Tonsil, bone marrow.
Synonyms :
CD34, CD34 molecule, CD34 antigen, HPCA1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.