product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ GJB2 / CX26 / Connexin 26 Antibody (Cytoplasmic Domain, clone CX-12H10) LS-B5511
catalog :
LS-B5511
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CX-12H10
reactivity :
human, rat
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; rat
Stewart M, Plante I, Bechberger J, Naus C, Laird D. Mammary gland specific knockdown of the physiological surge in Cx26 during lactation retains normal mammary gland development and function. PLoS ONE. 2014;9:e101546 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B5511 image 1
Anti-GJB2 antibody IHC of rat intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
AntibodyID :
140060
AntibodyName :
LS-B5511
TargetSpecies :
Rat
Host Species :
Mouse
Product Name :
IHC-plus™ GJB2 / CX26 / Connexin 26 Antibody (Cytoplasmic Domain, clone CX-12H10) LS-B5511
Specificity :
Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.
ClonalityDesc :
Monoclonal
CloneName :
CX-12H10
AntibodyModification :
Unconjugated
AntigenModification :
Cytoplasmic Domain
PresentationDesc :
PBS, pH 7.4, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26. (4,5)
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
GJB2 / CX26 / Connexin 26
StandardGeneSymbol :
GJB2
gene family :
Ion Channel
Subfamily :
Connexin
Reactivity :
Mouse, Rat, Human
Usage :
ELISA (0.1 - 1 µg/ml), IHC, IHC-Fr, IHC-P (10 µg/ml), WB (1 µg/ml)
ShortWebDescription :
Connexin 26 antibody LS-B5511 is an unconjugated mouse monoclonal antibody to Connexin 26 (GJB2 / CX26) (Cytoplasmic Domain) from rat. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Cited in 1 publication.
UsageText :
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Immunohistochemistry (Frozen): 1-2 ug/ml. Western Blot: 1 ug/ml. Reactivity confirmed using extracts from mouse liver, mouse brain and rat brain.
Synonyms :
GJB2, Connexin 26, Connexin-26, DFNB1A, DFNA3, DFNB1, Gap junction protein beta 2, KID, HID, PPK, NSRD1, CX26, DFNA3A
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.