product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ GSK3B / GSK3 Beta Antibody (phospho-Ser9) LS-B55
catalog :
LS-B55
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:200; fig 3
  • western blot; human
Allodi I, Comley L, Nichterwitz S, Nizzardo M, Simone C, Benitez J, et al. Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS. Sci Rep. 2016;6:25960 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B55 image 1
Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
27095
AntibodyName :
LS-B55
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ GSK3B / GSK3 Beta Antibody (phospho-Ser9) LS-B55
Specificity :
This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, rat, frog, chicken, dog and zebrafish based on 100% homology for the immunogen sequence. Cross reactivity with GSK3B homologues from other sources has not been determined.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pSer9
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1-15 of human GSK3 beta.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
GSK3B / GSK3 Beta
StandardGeneSymbol :
GSK3B
gene family :
Protein Kinase
Subfamily :
GSK-3
Reactivity :
Human
Usage :
ELISA (1:10000 - 1:50000), IF (1:200 - 1:1000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:3000)
ShortWebDescription :
GSK3 Beta antibody LS-B55 is an unconjugated rabbit polyclonal antibody to human GSK3 Beta (GSK3B) (pSer9). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: LS-B55 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B55 was determined to be 5 ug/ml.
Synonyms :
GSK3B, GSK-3 beta, GSK3 beta, GSK3beta, GSK3beta isoform, TPKI, GSK 3beta, Tau protein kinase I, ZGSK-3beta
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.